Aminoallyl-dUTP-XX-Cy5
产品编号：JBS-NU-803-XX-CY5-S
规格：10μl(1mM)
价格：1588元
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产品说明：Aminoallyl-dUTP-XX-Cy5Excitation and Emission spectrum of Cy5；运输：冰袋；保存条件: store at -20 °CShort term exposure (up to 1 week cumulative) to ambient temperature possible.；保质期：12 months after date of delivery；分子式：C57H80N7O23P3S2 (free acid)；分子量：1388.33 g/mol (free acid)；精确分子量：1387.40 g/mol (free acid)；纯度：≥ 95 % (HPLC)；形态：sterile blue solution in 10 mM Tris-HCl；浓度：1.0 mM – 1.1 mMpH: 7.5 ±0.5；光谱特性：λexc 649 nm, λem 670 nm, ε 250.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)；应用：Incorporation into DNA/cDNA by- PCR with Taq polymerase in-house data- Nick Translation with DNAse I/ DNA Polymerase I in-house data；说明：Aminoallyl-dUTP-XX-Cy5 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine.Recommended Aminoallyl-dUTP-XX-Cy5/dTTP ratio for PCR and Nick Translation: 20-30% Aminoallyl-dUTP-XX-Cy5/ 80-70% dTTP；注意事项：Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal produdct yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.
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