Aminoallyl-dUTP-XX-ATTO-425
产品编号：JBS-NU-803-XX-425-S
规格：10μl(1mM)
价格：1845元
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产品说明：Aminoallyl-dUTP-XX-ATTO-425Excitation and Emission spectrum of ATTO 425；运输：冰袋；保存条件: store at -20 °CShort term exposure (up to 1 week cumulative) to ambient temperature possible.；保质期：12 months after date of delivery；分子式：C46H67N6O21P3 (free acid)；分子量：1132.97 g/mol (free acid)；精确分子量：1132.36 g/mol (free acid)；纯度：≥ 95 % (HPLC)；形态：sterile green-yellow solution in 10 mM Tris-HCl；浓度：1.0 mM – 1.1 mMpH: 7.5 ±0.5；光谱特性：λexc 439 nm,λem 485 nm, ε 45.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)；应用：Incorporation into DNA/cDNA by- PCR with Taq polymerase in-house data- Nick Translation with DNAse I/ DNA Polymerase I in-house data；说明：Aminoallyl-dUTP-XX-ATTO425 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine.Recommended Aminoallyl-dUTP-XX-ATTO425/dTTP ratio for PCR and Nick Translation: 20-30% Aminoallyl-dUTP-XX-ATTO425/ 80-70% dTTP (PCR), 30-50% Aminoallyl-dUTP-XX-ATTO425/ 70-50% dTTP (Nick Translation)；注意事项：Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.
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