Aminoallyl-dUTP-XX-ATTO-594
产品编号：JBS-NU-803-XX-594-S
规格：10μl(1mM)
价格：1845元
https://aimg8.dlssyht.cn/u/931665/product/355/709866_1617156050.png?t=8739
产品说明：Aminoallyl-dUTP-XX-ATTO-594excitation and emission spectrum of ATTO 594；运输：冰袋；保存条件: store at -20 °CShort term exposure (up to 1 week cumulative) to ambient temperature possible.；保质期：12 months after date of delivery；分子式：C65H87N8O25P3S2 (free acid)；分子量：1537.48 g/mol (free acid)；精确分子量：1536.44 g/mol (free acid)；纯度：≥ 95 % (HPLC)；形态：sterile red-violet solution in 10 mM Tris-HCl；浓度：1.0 mM – 1.1 mMpH: 7.5 ±0.5；光谱特性：λexc 602 nm,λem 626 nm, ε 120.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)；应用：Incorporation into DNA/cDNA by- PCR with Taq polymerase in-house data- Nick Translation with DNAse I/ DNA Polymerase I in-house data；说明：Aminoallyl-dUTP-XX-ATTO594 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine.Recommended Aminoallyl-dUTP-XX-ATTO594/dTTP ratio for PCR and Nick Translation: 30-50% Aminoallyl-dUTP-XX-ATTO594/ 70-50% dTTP；注意事项：Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.
原网址：http://www.sima-lab.com/pro_21883259.html