DBCO-Sulfo-NHS Ester 10mg
价格:1900元
产品说明:订货编号:CCT-A124-10;规格:10mg;分子量:532.50;分子式:C25H21N2NaO8S;CAS NO.:1400191-52-7;溶解性:Water, DMSO, DMF;纯度:>95% (HPLC) ;外观:White to slightly grey crystalline;保存: -20°C. Desiccate ;运输要求:常温;简介:DBCO-Sulfo-NHS Ester Ester is water-soluble reagent that enables simple and efficient labeling of antibodies, proteins and any other primary amine-containing macromolecules with DBCO moiety in 100% aqueous buffers. It reacts with primary amines (e.g., side chain of lysine residues or aminosilane-coated surfaces) at neutral or slightly basic pH to form covalent bonds. DBCO-Sulfo-NHS Ester is a reagent of choice for applications that cannot tolerate organic co-solvents or are complicated by their inclusion. Specific labeling of cell surface proteins is another common application for these uniquely water-soluble and membrane impermeable reagents. DBCO reagents, also known as ADIBO or DIBAC, are the most commonly used substrates for strain-promoted click chemistry reaction. DBCO compounds react with azide functionalized compounds or biomolecules without the need for a Cu(I) catalyst to result in a stable triazole linkage. Protein?name Protein molecular weight (Da) Protein concentration (mg/mL) Volume of protein to be labeled (?L) Enter only one — either volume or amount of protein to be labeled Amount of protein to be labeled (mg) Molecular weight of labeling reagent (Da) Amount of labeling reagent used (mg) Maximum concentration of DMSO (%) * * Leave blank or 0 if not used Enter only one — either maximum concentration of DMSO or volume DMSO added to label Volume of anhydrous DMSO added (?L) Molar equivalents of labeling reagent used Volume of DMSO required to make a stock solution (?L) Volume of protein to be labeled (?L) Amount of stock labeling reagent to be added (?L) Amount of DMSO in labeling reaction (%) CoA Lot 2106 CoA Lot 2250 CoA Lot 2543″
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